diff --git a/Tests/test_file_apng.py b/Tests/test_file_apng.py
index 90759cdb551..fb47b53533e 100644
--- a/Tests/test_file_apng.py
+++ b/Tests/test_file_apng.py
@@ -353,6 +353,27 @@ def test_apng_save_different_palettes(tmp_path: Path) -> None:
         assert reloaded.mode == "RGBA"
 
 
+@pytest.mark.parametrize("mode", ("I;16", "I;16B"))
+def test_apng_save_i16(mode: str, tmp_path: Path) -> None:
+    test_file = tmp_path / "temp.png"
+    im = Image.new(mode, (1, 1), 1000)
+    second = Image.new(mode, (1, 1), 2000)
+    im.save(
+        test_file,
+        save_all=True,
+        append_images=[second],
+        duration=100,
+    )
+
+    with Image.open(test_file) as reloaded:
+        assert isinstance(reloaded, PngImagePlugin.PngImageFile)
+        assert reloaded.n_frames == 2
+        assert reloaded.getpixel((0, 0)) == 1000
+
+        reloaded.seek(1)
+        assert reloaded.getpixel((0, 0)) == 2000
+
+
 def test_apng_chunk_errors() -> None:
     with Image.open("Tests/images/apng/chunk_no_actl.png") as im:
         assert isinstance(im, PngImagePlugin.PngImageFile)
diff --git a/src/PIL/PngImagePlugin.py b/src/PIL/PngImagePlugin.py
index fdbd0bb659c..597931afcc9 100644
--- a/src/PIL/PngImagePlugin.py
+++ b/src/PIL/PngImagePlugin.py
@@ -1233,30 +1233,15 @@ def _write_multiple_frames(
 
             if im_frames:
                 previous = im_frames[-1]
-                prev_disposal = previous.encoderinfo.get("disposal")
-                prev_blend = previous.encoderinfo.get("blend")
-                if prev_disposal == Disposal.OP_PREVIOUS and len(im_frames) < 2:
-                    prev_disposal = Disposal.OP_BACKGROUND
-
-                if prev_disposal == Disposal.OP_BACKGROUND:
-                    base_im = previous.im.convert("RGBA")
-                    box = previous.bbox or (0, 0, *im.size)
-                    dispose = Image.new("RGBA", (box[2] - box[0], box[3] - box[1]))
-                    base_im.paste(dispose, box)
-                elif prev_disposal == Disposal.OP_PREVIOUS:
-                    base_im = im_frames[-2].im
+                if mode.startswith("I;16"):
+                    delta = ImageChops.subtract_modulo(
+                        Image.frombytes("LA", im.size, im_frame.tobytes()),
+                        Image.frombytes("LA", im.size, previous.im.tobytes()),
+                    )
                 else:
-                    base_im = previous.im
-                delta = ImageChops.subtract_modulo(
-                    im_frame.convert("RGBA"), base_im.convert("RGBA")
-                )
+                    delta = ImageChops.subtract_modulo(im_frame, previous.im)
                 bbox = delta.getbbox(alpha_only=False)
-                if (
-                    not bbox
-                    and prev_disposal == encoderinfo.get("disposal")
-                    and prev_blend == encoderinfo.get("blend")
-                    and "duration" in encoderinfo
-                ):
+                if not bbox and "duration" in encoderinfo:
                     new_duration = (
                         previous.encoderinfo["duration"] + encoderinfo["duration"]
                     )